The study by insurance brokers Hoden found that clubs paid a sky-high injury price of 513 million pounds ($550 million) last season.
The injury cost was calculated by multiplying the player’s daily cost by the number of days he was unavailable due to the injury.
FIFPro says structures must be put in place to reduce players’ workloads.
For the first time this year, a European campaign by way of the World Cup will be halted mid-season, causing more crowding in matches on both sides of the tournament in Qatar, which begins on November 20.
UEFA has increased the number of competitive matches with the addition of the Nations League to the international schedule and the expansion of European club competitions from 2024.
FIFA is set to follow suit, with the World Cup expanded to 48 teams from 2026.
The Premier League had the highest injury cost of any of the top five divisions at around £185m, with La Liga coming in second with a £109m margin.
French champions Paris Saint-Germain had the highest injury cost of any club in Europe at £34m.
Injuries in the Premier League reached 1,231 in 2021-2022, compared to 938 in the previous season.
The permanent adoption of five substitutions per side by football lawmakers IFAB is designed to reduce the burden on players.
But FIFPro is urging FIFA to move forward by imposing minimum four-week off-season breaks and a two-week mid-season break for each player.